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Journal of Pharmacology and Toxicology
Year: 2010  |  Volume: 5  |  Issue: 4  |  Page No.: 146 - 151

In vitro Toxicity of Multi Wall Carbon Nanoparticles on Hep G 32 Liver Cell Lines

R.N.R. Anreddy, N.R. Yellu, R.K. Devarakonda and H. Vurimindi    

Abstract: The aim of the present study was to evaluate the in vitro toxicity of two Multi Wall Carbon Nanotubes (MWCNT) on human hepatocytes (Hep G 32 cell lines). The toxic effects of carbon nanoparticles were analyzed after 24 h of incubation with different cell lines using MTT assay and also estimated the levels of Lactate Dehydrogenase (LDH) (that is leakaged into the media). The results of the LDH estimation demonstrated that exposure of multi wall carbon nanotubes to hepatocytes (Hep G 32) for 24 h resulted in concentration-dependent increase in LDH leakage and exhibited a significant (p<0.05) cytotoxicity at a concentration range of 3-50 μg mL-1. The TC50 or IC50 values (toxic concentration 50 i.e., concentration of particles inducing 50% cell mortality) of two nanoparticles (1) were found in the range of 36.99-37.15 μg mL-1, (2) were less than that of quartz (known toxic agent, 39.85 μg mL-1), indicating their cytotoxicity of carbon nanoparticles.

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