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Journal of Plant Sciences
Year: 2012  |  Volume: 7  |  Issue: 5  |  Page No.: 194 - 200

Antioxidant and Antibacterial Activity of Hippophae rhamnoides Methanolic Leaf Extracts from Dry Temperate Agro-climatic Region of Himachal Pradesh

N.S. Gill, R. Sharma, R. Arora and M. Bali    

Abstract: The present study was carried out to evaluate the antioxidant and antibacterial activity of methanolic leaf extract of Hippophae rhamnoides L. (Sea buckthorn, SBT) leaves under in vitro conditions. The chemical composition of the methanolic leaf extract was quantified in terms of total phenol and flavonoid contents. The Hippophae rhamnoides methanolic leaf extract exhibited potent antioxidant activity determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and then by 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), assays. The scavenging capacity by DPPH and ABTS of the extracts was found to increase with every unit increase of Hippophae rhamnoides leaf extracts reaching 68% at 30 μg mL-1 concentration. Further, the extract was evaluated for antibacterial activity against three human pathogenic bacteria E. coli, A. protophormial and M. luteus, however, it was maximum against E. coli. The study shows that the methanolic extract of Hippophae rhamnoides leaves have marked antioxidant and antibacterial activities.

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