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Journal of Plant Sciences
Year: 2011  |  Volume: 6  |  Issue: 5  |  Page No.: 202 - 212

Inter Simple Sequence Repeats Reveal Significant Genetic Diversity Among Chickpea (Cicer arietinum L.) Genotypes

Himanshu Aggarwal, Alka Rao, J.S. Rana, Jasbir Singh, Anil Kumar, Vinod Chhokar and Vikas Beniwal    

Abstract: The present investigation was carried out to access the suitability and reliability of ISSR analysis for inferring genetic diversity among chickpea genotypes. A total of six ISSR markers were used to study genetic diversity among 115 genotypes of chickpea. Out of six ISSR markers (GTGC)4 (GTG)5 and (TCC)5 were found to generate reproducible DNA fragments of size ranging from 0.15-3, 0.40-2.5 and 0.35-2 kb and yielded 2-9, 3-10 and 4-11 number of fragments, respectively. Clustering analysis separated all the genotypes into five genetically similar groups. The value of cophenetic correlation coefficient (r) is 0.91 which is considered as a good fit. The genetic similarity coefficient among 115 chickpea genotypes ranged from 0.01 to 0.90. These values indicated high genetic variability among chickpea cultivars and can be used efficiently for gene tagging and genome mapping of crosses to introgress the favourable traits such as high yield potential, disease and insect resistance into the cultivated genotypes.

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