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Journal of Plant Sciences
Year: 2009  |  Volume: 4  |  Issue: 4  |  Page No.: 114 - 121

Molecular Characterization of the Waxy Gene in Einkorn Wheat

Ya-Xi Liu, Wei Li, Yu-Ming Wei, Guo-Yue Chen and You-Liang Zheng    

Abstract: This study characterizes 15 waxy genes from 15 accessions of the einkorn wheats Triticum urartu, T. boeoticum and T. monococcum. The mature protein coding sequences of waxy genes were analyzed. Nucleotide sequence variations in these regions resulted from base substitution and/or indel mutations. This work identified 8 distinct haplotypes from the diploid wheat waxy gene sequences. A main haplotype was found in 7 gene samples from the Au genome and Am genome. The waxy gene sequences from the Au and Am genomes could be obviously clustered into two clades, but the sequences from the Am genome of T. boeoticum and T. monococcum could not be clearly distinguished. The phylogenetic analysis revealed that the waxy gene sequences from the Am genome had accumulated fewer variations and evolved at a slower rate than the sequences from the Au genome. These results would contribute to the understanding of functional aspects and efficient utilization of waxy genes.

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