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Journal of Plant Sciences
Year: 2008  |  Volume: 3  |  Issue: 1  |  Page No.: 111 - 115

Micropropagation of Baliospermum montanum (Willd.) Muell.-Arg.-A Red Listed Medicinal Plant

Satheesh George, S.P. Geetha and Indira Balachandran    

Abstract: A protocol has been standardized for mass in vitro propagation of Baliospermum montanum. Among the cytokinins, BA was superior to kinetin in terms of the number of shoots produced per explant. All the media containing cytokinins and auxins in combination showed superior results than the media with cytokinins alone. Murashige and Skoog (MS) medium containing 1.0 mg L1 BA in combination with 0.1 mg L1 NAA was superior for shoot multiplication. In this medium an optimum number of 9.25 shoots per explant was produced after six weeks of incubation. Among the auxins tried for root induction, NAA and IAA were the most and least effective in root induction, respectively. MS medium containing 0.2 mg L1 NAA was superior for root induction with an average number of 6 roots per shoot. Rooted plantlets on transfer to moist sand, got established successfully in one week and were planted out to pots containing sand, soil and cow dung in 1:1:1 ratio. The micropropagated plants were successfully established with 90 percent success.

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