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Journal of Plant Sciences
Year: 2007  |  Volume: 2  |  Issue: 6  |  Page No.: 635 - 639

Phosphate Solubilization by Rhizobium Isolates from Crotalaria Species

M. Sridevi, K.V. Mallaiah and N.C.S. Yadav    

Abstract: Rhizobium species isolated from Crotalaria species (C. junceaC. laburnifoliaC. retusa and C. verrucosa) were tested for their ability to solubilize tricalcium phosphate (TCP). Among these four species, Rhizobium sp. isolated from Crotalaria retusa and C. verrucosa showed zone of TCP solubilization on Pikovskaya`s agar medium. Highest solubilization index (2.70) was observed in Rhizobium sp. from C. retusa. Effect of different carbon and nitrogen sources was also studied. Among the carbon sources, glucose was found to be best carbon source for TCP solubilization. Presence of highest concentration (3%) of glucose enhanced TCP solubilization. Among the nitrogen sources, ammonium sulphate was the best promoter for the process. Addition of low levels (0.5µg mL-1) of EDTA increased TCP solubilization.

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