In vitro Effect of Various Growth Hormones in Capsicum annuum L. On the Callus Induction and Production of Capsaicin
The present study was aimed to develop a novel protocol for the in vitro induction of callus for the production of capsaicin from Capsicum annuum L. For callus production young leaves, growing shoots, nodal region from the sterile germinated seedlings and placental regions and pericarp tissue from the fruit pods were used as explants. They were cultured on MS Medium supplemented with the various combinations of GA, IAA, NAA, 2, 4-D and Kin. Of all tried combinations of growth hormones, MS Medium with 2.0 mg L1 2, 4-D and 0.5 mg L1 Kin was producing significant callus induction and proliferation in placental explants. The placental callus extract was taken for the estimation of capsaicin by colorimetric method. Extract had 1.6 mg1 of capsaicin g1 fresh weight of the callus. This could be an efficient protocol for capsaicin production from the placental calli and used for the large scale commercial production of capsaicin.
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