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Journal of Plant Sciences
Year: 2007  |  Volume: 2  |  Issue: 5  |  Page No.: 530 - 537

Protocol Establishment for Multiplication and Regeneration of Ocimum sanctum Linn. An Important Medicinal Plant with High Religious Value in Bangladesh

L.A. Banu and M.A. Bari    

Abstract: The experiment was conducted to Plant Biotechnology Laboratory of Institute of Biological Sciences, Rajshahi, Bangladesh. Shoot tip and leaf explants of Ocimum sanctum Linn. were cultured on different concentrations and combinations of growth regulators (BAP, Kin, 2, 4-D, IAA and IBA) in MS medium to observe shoot multiplication, callus induction, callus regeneration and root induction. Among the different concentrations and combinations of growth regulators, the highest percentage of shoot formation and highest average number of shoots were observed 90 and 5.88%, respectively in 0.2 mg L-1 BAP from shoot tip explants. Callus induction was obtained within 12-15 days of culture from leaf explants. The highest frequency (90.00%) of organic callus induction was observed in MS medium containing 1.0 mg L-1 NAA. Shoot regeneration occurred when the calli were sub cultured in Ms medium supplemented in BAP formulation. The highest percentage of shoot regeneration was obtained 90.00 in 0.2 mg L-1 BAP. In vitro grown shoots rooted best on MS medium containing 0.1 mg L-1 NAA. The in vitro grown plant lets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.

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