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Journal of Plant Sciences
Year: 2007  |  Volume: 2  |  Issue: 4  |  Page No.: 407 - 415

Establishment of Cell Suspension Culture and Plantlet Regeneration of Brinjal (Solanum melongena L.)

M.J. Hossa in, M. Rahman and M.A. Bari    

Abstract: The aim of this study to show, an efficient protocol for establishment of cell suspension culture and plantlet regeneration through cell culture from the cotyledonary explants of Brinjal (Solanum melongena L.). In this investigation, three varieties of Brinjal cv. Loda, China and Jhotika were used. In first step, the somatic embryogenic calli formation was done using MS medium supplemented with different concentrations of auxin and cytokinin singly or in combination. Cells of the three varieties were isolated from the rapidly growing embryogenic and friable calli using orbital shaker. For callus induction the isolated cells were transferred to MS liquid medium containing different hormonal concentrations and after 37-63 days of incubation the micro-calli were appeared. The Loda and China varieties showed the best result (8.0 and 8.2%, respectively) in 2 mg L-1 NAA+0.05 mg L-1 BAP and 2 mg L-1 2,4-D+0.05 mg L-1 BAP. For embryo formation, micro-calli were subcultured on MS solid medium and the Loda variety showed the best result (21%) in the medium containing 1.0 mg L-1 BAP+0.05 mg L-1 GA3. The bipolar embryos were selected and cultured in MS medium with different combinations and concentrations of auxin (NAA) and cytokinin (BAP and IBA) for shoot and root formation. Optimum shoot and root formations were recorded in MS medium supplemented with 0.75 mg L-1 NAA+1.5 mg L-1 BAP and 2.0 mg L-1 NAA+0.5 mg L-1 IBA, respectively. The plantlets appeared in the embryo mass were cultured and acclimatized.

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