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Journal of Plant Sciences
Year: 2006  |  Volume: 1  |  Issue: 1  |  Page No.: 72 - 79

A Novel Peroxidase from Withania somnifera

S. Johri, U. Jamwal, P. Kaiser, S.A. Tasduq, S. Rasool, V. Verma and G.N. Qazi    

Abstract: This study reports the isolation of a robust class 111 peroxidase (EC. from roots of Withania somnifera (WSPOD), which has the ability to oxidize indole-3-acetic acid (IAA) to free radicals like indolyl, skatolyl and peroxyl radicals which are highly cytotoxic species. WSPOD is not toxic when incubated independently in the assay system at equivalent concentrations. WSPOD, a monomer glycoprotein of 34 kDa and pI 5.2 is a highly thermotolerant enzyme with optimum pH ~6.0 and stable in the pH range of 3-9. Km and Vmax for IAA was calculated to be 0.17 mmol and 0.66 μmol min-1, respectively. The enzyme did not require H2O2 for the activity but needed 2,4-DCP and manganese ions as cofactors.

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