Plant Regeneration from Axillary Shoot Segments Derived Callus in Hemidesmus indicus (L.) R. Br. (Anantamul) an Endangered Medicinal Plant in Bangladesh
Callus cultures were obtained from axillary shoots of Hemidesmus indicus on (MS) medium supplemented with 2,4-D, NAA, IAA and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg L-1 NAA and 2.0 mg L-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (90.00%) from axillary shoot derived callus on MS medium containing 1.0 mg L-1 NAA and 2.5 mg L-1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
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