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Journal of Plant Sciences
Year: 2006  |  Volume: 1  |  Issue: 1  |  Page No.: 42 - 48

Plant Regeneration from Axillary Shoot Segments Derived Callus in Hemidesmus indicus (L.) R. Br. (Anantamul) an Endangered Medicinal Plant in Bangladesh

N.A. Siddique and M.A. Bari    

Abstract: Callus cultures were obtained from axillary shoots of Hemidesmus indicus on (MS) medium supplemented with 2,4-D, NAA, IAA and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg L-1 NAA and 2.0 mg L-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (90.00%) from axillary shoot derived callus on MS medium containing 1.0 mg L-1 NAA and 2.5 mg L-1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.

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