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Research Journal of Parasitology
Year: 2016  |  Volume: 11  |  Issue: 3  |  Page No.: 67 - 72

Diagnosis of Sheep Trichostrongylosis Based on Imune Response Profile in Experimental Rabbit Infection

Soad E. Hassan, Sanaa K.A. Abou-El-Dobal and Eman H. Abdel-Rahman    

Abstract: Background and Objective: Trichostrongylus colubriformis is a common nematode which infect livestock and can cause accidental infection in humans. In the present study, affinity purification process succeeded in the isolation of a single specific fraction identified by experimentally infected rabbit sera. Methodology: The isolated fraction detected T. colubriformis antibodies in 68 (77.3%) out of 88 examined sheep serum samples. By SDS-PAGE, the isolated fraction showed simple electrophoretic profile and resolved into three bands of molecular weights 74, 85 and 97 kDa. While eight bands of molecular weights ranged from 25.7-189 kDa were detected in crude adult worm extract. In immunoblot assay, the three bands of isolated fraction were reacted with infected rabbit serum compared with four bands of molecular weights 41, 74, 85 and 97 kDa in crude extract. Results: Concerning, humeral immune responses of infected rabbits, the level of IgG antibodies increased to reach (0.526 OD±0.0027) in the first-week post infection. Then decreased to reach (0.34 OD±0.02) at the end of the experiment using ELISA. A significant expression of IL4 was observed and reached the highest level (225±0.012) at second-week post infection. While, IFN-ɣ showed less and nearly constant expression through out the time of the experiment. Conclusion: The partially immuno-affinity purified fraction of T. colubriformis adult antigen proved high diagnostic efficacy in diagnosis of trichostrongylosis in sheep. In addition, T. colubriformis infection stimulates rabbits cellular and humeral immune responses IL4, IFN-γ and IgG which, possibly create some level of protection to re infection.

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