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Research Journal of Parasitology
Year: 2016  |  Volume: 11  |  Issue: 1-2  |  Page No.: 13 - 19

Diagnosis of Toxoplasmosis Using Affinity Purified Fraction of Tachyzoites Local Isolate

Soad E. Hassan, Nagwa I. Toaleb, Raafat M. Shaapan, Eman Hussien Abdel-Rahman and Ehab Kotb Elmahallawy    

Abstract: To increase diagnostic potency of antigen, its immunogenic component must be isolated. Immuno-affinity column chromatography was adopted to purify immunogenic fraction of Toxoplasma gondii tachyzoites of chicken local isolate. The isolated fraction was characterized by SDS-PAGE, where it exhibited 2 bands of molecular weights 116 and 83 kDa compared with 8 bands of molecular weights ranged from 116-30 kDa in crude antigen. The isolated fraction proved high diagnostic activity compared with crude antigen by ELISA using two fold serial dilutions of infected human and chicken sera. The fraction detected antibodies in 366 out of 406 (90.1%) examined free range chicken serum samples. Also, it proved success in the detection of Toxoplasma antibodies in 30 serum samples of patient infected with T. gondii. The fraction proved 100% sensitivity and 84.4% specificity. Its Positive Predictive Value (PPV), Negative Predictive Value (NPV) and diagnostic accuracy were 85.7, 100 and 91.9%, respectively. In conclusion, the present study introduced novel T. gondii chicken tachyzoites partially purified fraction proved high diagnostic activity of chicken and human toxoplasmosis.

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