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Journal of Medical Sciences
Year: 2018  |  Volume: 18  |  Issue: 3  |  Page No.: 108 - 113

Expression of MSH2 in Head and Neck Lymphomas (A Study Utilizing Immunohistochemistry and Real-time Polymerase Chain Reaction)

Safa Fathy Abd El-Ghani, Rehab Fawzi Kasem, Dina Soliman Khater and Olfat G. Shaker    

Abstract: Background and Objective: Lymphomas are malignant neoplastic proliferations that cause major health problems worldwide. However, the precise pathogenesis and underlying molecular mechanisms of both Hodgkin’s lymphoma (HL) and non-Hodgkin’s lymphoma (NHL) are not fully elucidated. Accordingly, the aim of the present study was to detect the expression of MSH2 at the gene and protein levels in HL and NHL of the head and neck. Materials and Methods: Expression of MSH2 on gene and protein levels were detected using quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry respectively. A total of 26 archival specimens were selected including normal lymph nodes (6 cases) which were used as a control group, lingual tonsils with lymphoid hyperplasia (6 cases) and 14 cases of lymphomas of submandibular and sublingual lymph nodes. Lymphomas were classified into of HL (6 cases of mixed cellularity type) and NHL (8 cases of diffuse B cell lymphoma). Results: Lower expression of MSH2 in NHL than HL was also observed. Moreover, a positive correlation was detected between the expression of MSH2 protein and its mRNA among the examined groups. Conclusion: Lower expression of MSH2 in NHL than that of HL suggested a more aggressive behavior and poor prognosis in this lesion. The reduced expression of MSH2 could be considered as a molecular biomarker for loss of MMR activities and it might serve as a prognostic biomarker for head and neck lymphomas.

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