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Journal of Medical Sciences
Year: 2015  |  Volume: 15  |  Issue: 5  |  Page No.: 221 - 228

Simultaneous Determination of Paracetamol, Guaiphenesin and Chlorpheniramine Maleate Using Ultraviolet Spectroscopy in Combination with Multivariate Calibration

Abdul Rohman, Yani Ardiyanti, Sudjadi and Sugeng Riyanto    

Abstract: The method of choice for analysis of drugs in multi-component preparations is chromatographic based technique such as High Performance Liquid Chromatography (HPLC). However, chromatographic method is time consuming and requiring much effort. As a consequence, some simple methods such as UV spectrophotometry are continuously developed, especially in combination with the chemometrics software. The UV-vis spectrophotometry coupled with multivariate calibration of Partial Least Square (PLS) has been developed for quantitative analysis of paracetamol, guaiphenesin and chlopheniramine maleate in the presence of phenylpropanolamine without separation step. The calibration model is prepared by developing a series of sample mixture comprising these drugs in certain proportion. The evaluation of calibration model was based on coefficient of determination (R2) and Root Mean Square Error of Calibration (RMSEC). The result showed that UV spectrophotometry combined with PLS can be used for quantitative analysis of drugs. The coefficient of determination (R2) for the relationship between actual values and predicted values was higher than 0.99 indicating good accuracy. The RMSEC values obtained were relatively low indicating good precision. The accuracy of developed method was compared to that of HPLC. The developed method was successfully used for analysis of paracetamol, guaiphenesin and chlopheniramine maleate in tablet dosage form.

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