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Journal of Medical Sciences
Year: 2012  |  Volume: 12  |  Issue: 7  |  Page No.: 244 - 247

Detection of Cefotaximase Genes of Beta Lactamase among Clinical Isolates of Escherichia coli in a University Teaching Hospital, Nigeria

C.N. Akujobi, C.C. Ezeanya and N.I. Aghanya    

Abstract: Cefotaximase genes of Beta Lactamase have been responsible for community acquired infections such as urinary tract infections. Presently, organisms harbouring these genes are spreading across hospitals world wide. The presence of these genes among Escherichia coli has posed serious therapeutic problems. In this study, cefotaximase genes of beta lactamase among clinical isolates of Escherichia coli were detected using polymerase chain reaction. We also investigated the antibiotic susceptibility pattern of this organism from June-November 2012. Clinical isolates from patients were collected at the bacteriology laboratory of a university teaching hospital. The isolates were subjected to proper identification using standard microbiological techniques. Antibiotic susceptibility testing was done using the Disc Diffusion Method. Amongst the isolates of E. coli collected, 100% susceptibility to one or more antimicrobial agent(s) by all the isolates was not observed. An average of 30-65 isolates of E. coli showed intermediate susceptibility to complete resistance to all the antimicrobial agents. It is of clinical interest that the E. coli susceptibility pattern observed in this study is incorporated in the available antibiotic susceptibility profile of E. coli. However, high rate of resistance to third generation cephalosporins, aztreonam and ciprofloxacin observed was disturbing. Isolates harbouring Cefotaximase (CTX-M) genes showed more resistance to cefotaxime. There was 53% prevalence rate of Cefotaximase gene among the isolates. Nevertheless, the continuous monitoring of antibiotic susceptibility pattern of E. coli in Nigerian hospitals is needed to assess the challenge regarding drug resistance, which will allow doctors to adjust the empirical treatment.

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