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Journal of Medical Sciences
Year: 2007  |  Volume: 7  |  Issue: 5  |  Page No.: 724 - 731

In vivo the Immunological Effects of Fasciola gigantica Worms Homogenate Mixed with Saponin on Mice Infected with Schistosoma mansoni

S.A. Maghraby, Kamel H. Shker, H.G. Zahran and Mahmoud El-Sherbiny    

Abstract: Antischistosomal activity, identification and immunoprophylatic effects of a crude Faciola gigantica worms homogenate, F. gigantica worms homogenate mixed with saponin were studied on Schistosoma mansoni infected mice. Mice were divided into four groups (10 animals group). First group was immunized subcutaneously (50 μg/mouse/100 μL/PBS) at 0 and 15 days with a crude F. gigantica worms homogenate, second group was immunized subcutaneously with a crude F. gigantica worms homogenate mixed with saponin and, third group was immunized with saponin only. The fourth group was used as control. Post second immunization mice were infected by tail immersion with 100 Schistosoma mansoni cercariae per each mouse. Relative to electrophoretic analysis and scanning of the standard of known molecular weights, the number of major bands detected from electrophoretic scanning of Faciola gigantica worms homogenate were 8 bands, 177.30, 156.81, 110, 105.95, 102.47, 64.084, 56.403 and 45.893 KDa. The electrophoretic scanning of a crude Faciola gigantica worms homogenate mixed with saponin showed that 7 bands, 120.49, 112.38, 102, 91.727, 86.203, 69.794 and 66.825 kDa. On the other hand, the number of major bands detected from the electrophoretic scanning of saponin were 9 bands, 167.68, 110, 104.94, 102, 92.620, 79.399, 60, 54.231 and 23.091 KDa. Perfusion and recovery of adult worms were performed at 8 weeks post infection. Immunization with a crude F. gigantica worms homogeneta, a crude F. giganatica worms homogeneta mixed with saponin and saponin only recorded reduction in total worms by 75.11, 45.24 and 87.33%, respectively. The level of both IgM and IgG from sera of immunized mice with a crude F. gigantica worms homogenate, a crude F. gigantica worms homogenate mixed with saponin and saponin only showed an increasing against cercarialantigen preparation, soluble worm antigen preparation and soluble egg antigen using Enzyme Linked Immunosorbent Assay (ELISA). Using immunolymphocytes stainingpreparation, the mean number of splenocytes that were prepared from immunized groups with a crude F. gigantica worms homogenate, a crude F. gigantica worms homogenate mixed with saponin and saponin without homogenate , respectively showed a significant increase (p<0.05) as compared with non immunized group.

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