Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Journal of Medical Sciences
Year: 2006  |  Volume: 6  |  Issue: 6  |  Page No.: 1015 - 1020

Detection of Full Length Hb S Gene (1.2 Kb) in One- and Two-cell Embryo Originated from Hamster Oocyte and Human Spermatozoa by Using Nested-PCR

Bahy A. Ali, Halima H. Salem, Tian H. Huang and Qing D. Xie    

Abstract: The polymerase chain reaction (PCR) is a versatile method for rapid amplification of selected DNA segments. The aim of this study was to amplify the full length of Hepatitis B Surface (HBS) gene in one- and two-cell embryo (single embryo) after introducing motile human sperm carrying hepatitis B virus (HBV) DNA into zona-free hamster oocytes via in vitro fertilization (IVF) technique. Human sperm-mediated HBV genome was delivered into zona-free hamster oocytes by IVF. The results revealed that full length HBS could be amplified in one-and two-cell embryo originated from hamster ova in vitro fertilized with human spermatozoa carrying HBV DNA sequences.

Cited References   |    Fulltext    |   Related Articles   |   Back
 
 
   
 
 
 
  Related Articles

 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility