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Journal of Medical Sciences

Year: 2004  |  Volume: 4  |  Issue: 4  |  Page No.: 318 - 322

Comparison of Liver Function Tests After 2/3 Hepatectomy and Liver Biopsy in Experimental Rats

Siddique M., S.A. Malik, A.S. Khan, G. Nazir and J.A. Chawla


Previous studies have shown that changes in liver function profile may vary with postoperative time interval and may be related to the extent of hepatic resection. This study describes the characteristic profiles in parenchymal liver enzymes and other serum liver function tests over a one-week course comparing 2/3 hepatectomy with liver biopsy (<10% resection) in experimental rats. A group of 6 animals was used for this purpose, separately for hepatectomy and biopsy, respectively. The hepatic venous blood samples were drawn at 4, 8, 16, 24, 36, 48, 72, 96 and 168 h, respectively. The tests included were alanine amino transferase (ALT) aspartate amino transferase (AST) alkaline phosphatase (ALP), lactate dehydrogenase (LDH), gamma glutamyl transpeptidase, total protein, serum albumin and bilirubin. The results were compared with normal control group of animals. Both serum ALT and AST showed early increase (8 h) after both operations. ALT returned to near control level between 48-72 h, while AST regained normal level by 72 h in both groups. Noticeable increase in ALP occurred only in the partially hepatectomized group with a delay between 24-96 h before ultimate normalization. No significant changes for ALP occurred in the biopsy group. LDH increased early in both experimental groups and the level tended to normalize by 168 h of surgery in the biopsy group while in hepatectomized group there was a steady decline towards control level. In contrast alkaline phosphatase and γ GT showed little change in partially hepatectomized group but in biopsy group noticeable increase occurred in comparison with control and hepatectomized group. The total protein dropped below the control level by about 25% at 4 h after partial hepatectomy. The decrease in biopsy group was little in the same period. Serum bilirubin remained above normal initially for 16 h and then became normal after 7 days in partial hepatectomy group. Interestingly the change in biopsy group was opposite to hepatectomy group point by point. The only tests that differ in two groups and seem to play a role in a clinical setting are serum bilirubin, total protein and γ GT.

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