Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Journal of Medical Sciences
Year: 2004  |  Volume: 4  |  Issue: 4  |  Page No.: 294 - 299

Avoiding Antibodies Interferences on Serum IgA Detection in Rheumatoid Arthritis

Medhat Haroun    

Abstract: The aim of this study was to investigate the presence of anti-immunoglobulin antibodies in patients with rheumatoid arthritis, using as a target goat immunoglobulins, in an attempt to elucidate further complex immuno-pathogenetic interactions of the disease. Serum immunoglobulin A concentrations were measured by ELISA technique in twenty patients with rheumatoid arthritis and twenty matched normal adult individuals. The effect of anti-goat immunoglobulin antibodies leads to an overestimation of serum IgA level in patients; this increase is different for each sample estimated by ELISA. Visualization of IgA by immunoblotting technique confirms that goat immunoglobulins were co-precipitated with the antibody-antigen complex. Initial results before purification from the interfering anti-goat immunoglobulin antibodies suggested that patients had increment levels of IgA in their sera. It was found that normal individuals had a mean IgA level of 2.77 mg mL-1 and patients had a mean IgA level of 5.13 mg mL-1 (p<0.00004). While, the mean level of IgA in patients after purification from anti-goat immunoglobulins was 2.92 mg mL-1. Therefore, there was no significant difference in IgA level in patients purified from anti-goat immunoglobulin antibodies compared to normal individuals (p<0.33). A circulating immunoglobulin reacting with other immunoglobulins is thus present in patients with rheumatoid arthritis and may well play a part in the complex immuno pathogenetic interactions.

Cited References   |    Fulltext    |   Related Articles   |   Back
  Related Articles

Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility