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Journal of Medical Sciences

Year: 2004  |  Volume: 4  |  Issue: 3  |  Page No.: 206 - 209

Diagnosis of Trichomonas vaginalis Infection by Urine PCR Analysis Compared to Wet Mount Microscopic Screening

B. Kazemi, F. Yasaee, M. Bandehpour, N. Seyed, Y. Mehrabi, M. Rajabnejad, M. Mansouri, S. Givrad, M. Ghazi and H Baseri

Abstract

This study was conducted for parasite detection in urine samples using Polymerase Chain Reaction (PCR) and comparing the results to wet mount microscopic screening. For this, 155 urine samples were collected from symptomatic women suspected to trichomoniasis (mean age = 20±5 year) introduced to laboratory by physician. Urine samples were subjected to polymerase chain reaction to detect the parasite's genome. As a result 25 out of 155 samples (16%) were detected positive by direct microscopic observation, but 75 out of 155 (48%) by PCR. Eighty out of 155 samples (52%) were detected negative by PCR. This study confirmed that the microscopic screening with a low sensitivity must be substituted by highly sensitive screening methods such as PCR.

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