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Journal of Medical Sciences

Year: 2004  |  Volume: 4  |  Issue: 2  |  Page No.: 132 - 135

Comparative Study on HCV Detection in Iranian Patients by RT-PCR and ELISA Techniques During 2001-2003

B. Kazemi, Bandehpour, H. Yahya zadeh, M. Roozbehi, N. Seyed, A. Ghotasloo and A. Taherpour

Abstract

HCV is one of the main causes of chronic hepatitis in developing countries and is particularly distributed via blood and blood product transfusion. ELISA serological method is currently used as laboratory diagnostic test in Iran, but both clinical and laboratory diagnosis of acute HCV infection remains yet as a problem. The aim of this study was to determine results obtained by PCR and ELISA techniques in detection of HCV in serum samples. Using ELISA and RT-PCR, 159 serum samples were analyzed. Thirty four negative samples (22.35%) and 45 positive samples (27.95%) were detected by both techniques. Thirty two samples (19.88%) were positive by PCR and negative by ELISA and 48 samples (29.82%) were negative by PCR and positive by ELISA. Present results show that there are significant differences between PCR and ELISA diagnostic tests for HCV detection.

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