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Journal of Molecular Genetics

Year: 2016  |  Volume: 8  |  Issue: 1  |  Page No.: 1 - 8

Identification, CDNA Cloning and Characterization of Pax3 Gene in Chinese Domestic Goose

Cui Wang, Yi Liu, Yunzhou Yang, Huiying Wang, Shaoming Gong, Weihu Chen and Daqian He

Abstract

Paired box (Pax) protein 3 is a member of the Pax family of transcription factors, plays critical roles in muscle development. Many studies showed that Pax3 gene was a functional candidate gene for production and meat quality. However, the sequence information of goose Pax3 gene remains unknown. In this study, the comparativegenomicstechnology was used to clone the cDNA sequence of Pax3 gene from the breast muscle tissue of Zhedong goose. The gene structures were analyzed by the bioinformatics software and the mRNA expression profile of Pax3 gene in different tissues was measured by the semi-quantitative RT-PCR. The goose Pax3 full-length coding sequence consisted of 1327 bp and encoded 421 amino acids. Sequence analysis displayed that a splicing variant of goose Pax3 absence of a glutamine residue was also identified. Both of the Pax3-a (inclusion of a glutamine residue) and Pax3-b (exclusion of a glutamine residue) isoforms were predicted contain three conserved domains (a paired domain, an octapeptide region and a homeodomain) and were highly conserved (>90%) relative to the known Pax3 proteins from other species. Multiple sequence alignments and phylogenetic analysis displayed that the deduced goose Pax3 proteins have a close genetic relationship and evolutional distance with the Pax3 proteins in other avian species, especially in ducks. The semi-quantitative RT-PCR results revealed Pax3 mRNA was highly expressed in the breast muscle tissue, followed by the lung, leg muscle and brain, little or no expression was observed in heart, liver, spleen, kidney, muscular stomach, intestine and sebum. These findings will help us understand the functions of the Pax3 gene and the molecular breeding in Chinese domestic goose.

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