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Journal of Electron Microscopy
Year: 2010  |  Volume: 59  |  Issue: 3  |  Page No.: 237 - 241

Cryo-electron tomography of Chlamydia trachomatis gives a clue to the mechanism of outer membrane changes

Z Huang, M Chen, K Li, X Dong, J Han and Q. Zhang    

Abstract:

Cryo-electron tomography was employed to reconstruct the structure of Chlamydia trachomatis. Results revealed that the features of the structures, especially those of the membranes, were preserved much better than those by conventional ultrathin section methods. This method also enabled us to determine that the thickness of the outer membrane of the elementary bodies is nearly twice of that of the reticulate bodies. Our observations give a clue to the mechanism of outer membrane changes.

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