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Research Journal of Microbiology
Year: 2015  |  Volume: 10  |  Issue: 10  |  Page No.: 452 - 465

Potential of Chitinolytic Bacillus amyloliquefaciens SAHA 12.07 and Serratia marcescens KAHN 15.12 as Biocontrol Agents of Ganoderma boninense

Siti Nur Azizah, Nisa Rachmania Mubarik and Lisdar I. Sudirman    

Abstract: The objective of this research was to screen and identify of chitinolytic bacteria which have an ability to inhibit the growth of G. boninense and also to characterize its chitinase activity on degrading the chitin of G. boninense. Screening of chitinolytic bacteria against G. boninense by using dual culture method on Potato Dextrose Agar (PDA) showed that bacterial isolates SAHA 12.07 and KAHN 15.12 had the highest percentage inhibition of 68,19 and 40, 29%, respectively. Based on 16S rRNA identifications, SAHA 12.07 and KAHN 15.12 were identified as Bacillus amyloliquefaciens and Serratia marcescens, respectively. Isolate SAHA 12.07 produced optimum chitinase at 48 h and KAHN 15.12 at 54 h of incubation in chitin medium. Crude chitinase of SAHA 12.07 and KAHN 15.12 have precipitated by acetone at 60 and 20%, respectively. Chitinase activity had active at pH 4-10 and temperature about 20-80°C. In vitro lysis of G. boninense bioassay showed that the chitinase of SAHA 12.07 and KAHN 15.12 were capable to inhibit the growth of mycelium G. boninense and could release of N-acetylglucosamine by incubation of G. boninense with partially purified enzyme.

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