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Research Journal of Microbiology
Year: 2015  |  Volume: 10  |  Issue: 1  |  Page No.: 24 - 32

Isolation and Identification of Antimicrobial Protein from Saccharomyces cerevisiae and its Efficacy Against the Human Pathogens

P. Senthilraja, Sunil Kumar Sahu and K. Kathiresan    

Abstract: Antimicrobial peptides (AMPs) are an important component of the natural defences of most living organisms against invading pathogens. In the present study marine yeast (Saccharomyces cerevisiae) was cultured in Yeast Malt Agar medium followed by protein extraction and quantification by using sonication method and Bradford assay, respectively. The isolated protein was then subjected for native PAGE and the four distinct bands were excised and tested for antibacterial activity against five bacterial human pathogens viz. Escherichia coli, Staphylococcus aureus, Vibrio cholera, Salmonella typhi and Shigella flexneri. Of the four protein bands, only one band exhibited antibacterial activity which was identified to have a molecular weight of 60 KDa. The peptides analysis was made through using the MASCOT search engine, using MALDI-TOF/MS result revealed that the peptides machinhg 51%, 10 peptides sequence was mached 60 KDa heat shock protein. the protein retrieve uniprot database total primary sequence 341 amino acids. The secondary structure of the identified protein indicated that the amino acids was mainly composed of helical form (49.85%) followed by the Coil structure (24.63%) and strand (14.08%). The identification of HPS60 heat shock proteins from marine yeast provides further evidence that this protein may play a pivotal role against a wide range of human pathogens.

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