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Research Journal of Microbiology
Year: 2012  |  Volume: 7  |  Issue: 2  |  Page No.: 139 - 144

Cultural and Morphological Characterization of Trichoderma spp. Associated with Green Mold Disease of Pleurotus spp. in Kashmir

Shaiesta Shah, Sahera Nasreen and P.A. Sheikh    

Abstract: Various micro fungi associated with Pleurotus spp. were isolated from Mushroom Research and Training Center (M.R.T.C) in SKAUST, Srinagar. Among various fungi collected, the most predominant were those of Trichoderma spp. A total of 49 isolates of Trichoderma spp. were collected during oyster mushroom cultivation. On the basis of cultural and morphological characteristics, Trichoderma isolates were classified into three species, namely Trichoderma harzianum, Trichoderma viride and Trichoderma pseudokoningii. The predominant specie was Trichoderma harzianum (n = 19) followed by Trichoderma viride (n = 17) and Trichoderma pseudokoningii (n = 13). All the three species were distinct from each other in the characteristics such as mycelial growth rate, colony appearance, shape of conidia and conidiophores and branching pattern of phialides. This study has found Trichoderma harzianum to be the main cause of green mold disease of Pleurotus spp.

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