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Research Journal of Microbiology
Year: 2012  |  Volume: 7  |  Issue: 1  |  Page No.: 1 - 12

Biological Pretreatment of Rice Straw by Phenarocheate chrysosporium for the Production of Cellulases and Xylanases using Asperigillus niger Isolate

P.R. Mohan, B. Ramesh and O.V.S. Reddy    

Abstract: Rice straw was pretreated biologically with Phenarocheate chrysosporium and subsequently used in the production of cellulases and xylanases employing Asperigillus niger isolate. The FTIR spectrum of pretreated rice straw was compared with standard cellulose library. The spectra of 1048 cm-1 for C-O stretch in cellulose and hemicellulose were observed in rice straw. The enzymatic activity produced by A. niger on biologically Pretreated Rice Straw (PRS) was higher than that of the control treatments, which were carried out with Untreated Rice Straw (URS) and Cellulose (CEL). For medium supplemented with PRS, the peak activities obtained were 1.03±0.06, 1.54±0.08, 0.82±0.14 and 1.02±0.08 U mL-1 for FPase, endoglucanase, β-glucosidases and xylanases, respectively. For the cellulose control, values of 0.68±0.04, 1.24±0.11, 0.54±0.16 and 0.90±0.06 U mL-1 and URS values of 0.48±0.08, 0.94±0.10, 0.46±0.12 and 0.64±0.14 U mL-1 were obtained, respectively. This was substantiated by higher contents of N-acetylglucosamine and fungal protein in PRS samples than the cellulose control and untreated rice straw samples.

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