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Research Journal of Microbiology
Year: 2010  |  Volume: 5  |  Issue: 10  |  Page No.: 980 - 985

Fungal Invertase as Aid for Production of Ethanol from Sugarcane Bagasse

C. Uma, C. Muthulakshmi, D. Gomathi and V.K. Gopalakrishnan    

Abstract: The present study is aimed at the production of bio-ethanol from two fungal strains with the addition of the enzyme invertase using sugarcane bagasse as the substrate. Optimization for production of ethanol from sugarcane bagasse was determined by enzymatic saccharification using fungal strains. Enzymatic hydrolysis of sugarcane bagasse and ethanol fermentation from lignocellulosic hydrolysate were investigated by three different methods namely separate hydrolysis and fermentation process, simultaneous saccharification and fermentation and co-culture methods. The enzyme invertase from two different fungal strains were supplemented and the ethanol yield was calculated. Result of the present study report growth optimization of two fungal strains capable of saccharifying sugarcane bagasse. The hydrolyzed material was then fermented with the help of yeast into ethanol. Result of the study reveals the ethanol production by C. cladosporioides was higher (48%) after 48 h of fermentation under static condition by using submerged fermentation.

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