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Research Journal of Microbiology
Year: 2010  |  Volume: 5  |  Issue: 8  |  Page No.: 707 - 716

Studies on Selection of Efficient Bacterial Strain Simultaneously Tolerant to Hexavalent Chromium and Pentachlorophenol Isolated from Treated Tannery Effluent

M. Tripathi and S.K. Garg    

Abstract: Bioremediation is being viewed as a clean technology for decontamination of pentachlorophenol and chromium from tannery effluent. This study was conducted to isolate an efficient bacterial culture from treated tannery effluent which is tolerant to pentachlorophenol and Cr (VI) and could be employed for simultaneous bioremediation of both the toxic contaminants. Tannery effluent sample was collected from Common Effluent Treatment Plant, Kanpur (India). Fifty four bacterial cultures isolated by enrichment culture technique were screened for PCP and Cr (VI) tolerance on minimal salt medium supplemented with glucose as a cometabolite. The isolate B4 was found to be maximally tolerant to high concentration of both pentachlorophenol (500 mg L-1) and chromium (VI) (200 mg L-1) and was selected for further studies. It was identified as Bacillus sp. by morphological and biochemical analyses. The effect of various growth parameters such as carbon source at 0.2-1.0% (glucose, maltose, sucrose), pH (6.5-8.0), temperature (25-40°C) and inoculum size (0.5-2.5% v/v) were evaluated in minimal salt medium supplemented with 500 mg L-1 PCP and 200 mg L-1 Cr (VI). The best growth was exhibited at 0.4 % glucose, pH 7.0, 35°C and with 1.0% inoculum under shaking at 150 rpm. Thus, our isolate appears to have great potential for simultaneous bioremediation of pentachlorophenol and hexavalent chromium from the contaminated sites.

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