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Research Journal of Microbiology
Year: 2009  |  Volume: 4  |  Issue: 10  |  Page No.: 361 - 365

Viability of Antifungal Metabolite Producing Pseudomonas Bacteria

M.S. Shathele and A. Fadlelmula    

Abstract: The objectives of this study were to determine the suitability of transport medium (ice jells) and estimate the duration of viability of Pseudomonas in the transport medium. Bacteria of the genus Pseudomonas comprise a large group of the active biocontrol strains as a result of their general ability to produce a diverse array of potent antifungal metabolites. These include simple metabolites such as 2,4-diacetylphloroglucinol, phenazine-1-carboxylic acid and pyrrolnitrin [3-chloro-4-(2-nitro-3-chlorophenyl)-pyrrole], as well as the complex macrocyclic lactone, 2, 3-de-epoxy-2, 3-didehydro-rhizoxin. Pyrrolnitrin is active against Rhizoctonia sp., Fusarium sp. and other pathogenic fungi and it has been used as a lead structure in the development of a new phenylpyrrole fungicide. The survival rates of four different pseudomonad strains after continuous incubation for 4 h in the cold temperature (4°C) were: 94.8% for P. putida strain CBD, 94.5% for P. aeruginosa No. BRCH and 62.1% for Pseudomomas species (fluorescent) with lowest survival rate of 33.5% for P. aeruginosa strain H. Since, there were no drastic reductions in the survival rates, the study findings suggest that the transport medium would be generally suitable for these cold-sensitive bacteria.

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