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Research Journal of Microbiology
Year: 2009  |  Volume: 4  |  Issue: 5  |  Page No.: 186 - 193

Sero Diagnosis of Bluetongue virus Infection and Isolation of Virus in Embryonated Chicken Egg and BHK-21 Cell Line

N. Ramesh, V. Rajesh Kannan, K. Karthikeyan, K. Nanthakumar and R. Karthik Raja    

Abstract: Isolation of Bluetongue virus from blood samples of sheep and goat was carried out in the present study. Out of one fifty blood samples screened for seroprevalance of BTV antibodies by Agarose Gel Precipitation Test (AGPT) 42 gave positive results. The overall percentage of virus isolation was 28% from Embryonated Chicken Eggs (ECE). The identities of the isolates were confirmed by cytopathogenicity. All the isolates were passaged twenty one times in embryonated chicken eggs and further passaged in BHK-21 cell lines. The viral isolates adapted well to the cell culture system and produced cytopathic change like grouping of cells, polycaryon, syncytica formation, acidophilic and intracytoplasmic inclusion bodies in BHK-21 cells. This study confirms the BTV incidence in the tested blood sample with a possible means showing that the virus can easily adapt to ECE and BHK-21 cell line.

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