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Research Journal of Microbiology
Year: 2009  |  Volume: 4  |  Issue: 4  |  Page No.: 150 - 157

Spectra of Antibacterial Activity of Propolis (Promax-C) Samples from Two Localities of Adamaoua Province (Cameroon)

A. Mbawala, F.N. Tchuenguem Fohouo, D. Roger and J.B. Milliere    

Abstract: Fifteen samples of Promax-C, ethanolic extracts of propolis collected from different hives situated in two localities of the Adamaoua Province of Cameroon were tested each against seven strains of bacteria namely Samonella enterica, Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Listeria monocytogenes, Pseudomonas fluorescens and Bacillus subtilis. The aim of this study was to evaluate the antibacterial activity of those Promax-C samples. Antibacterial activity essays were investigated by the determination of the zones of growth inhibition using the well diffusion method on agar medium and the evaluation of the Minimal Inhibitory Concentration (MIC) using the macrodilution method. All the Promax-C samples were active against the Gram positive bacterial strains except E. faecalis. On the other hand, there was no activity of those samples on the Gram negative bacterial strains studied. Considering the diameter of the inhibitory zones and the MIC values, the susceptibility of bacterial strains to the Promax-C samples decreased as follows: L. monocytogenes>S. aureus>B. subtilis. The most active sample was Promax-C8 from the Martap locality and the most susceptible bacteria was L. monocytogenes. The areas of the minor and major peaks of the phenolic compounds obtained by HPLC analysis were more important for the Promax-C8 sample, showing that the greatest activity of these antimicrobial components was probably linked to their higher contents in the samples.

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