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Research Journal of Microbiology
Year: 2008  |  Volume: 3  |  Issue: 7  |  Page No.: 514 - 519

Characterisation of Extended Spectrum Beta-Lactamase Producing E. coli from Secondary and Tertiary Hospitals in South Eastern Nigeria

I.R. Iroha, M.U. Adikwu, E.S. Amadi, I. Aibinu and C.O. Esimone    

Abstract: Clinical isolates of Escherichia coli (No. = 109) were collected from Microbiology Laboratory unit of a tertiary hospital (Ebonyi State University teaching hospital, Abakaliki, EBSUTH) and a secondary hospital (Federal Medical Center, Abakaliki, FMC) from four different clinical specimens (urine, stool, blood and sputum) between February to November 2006. Sixty-three clinical isolates of E. coli were isolated from EBSUTH while forty-six were from FMC Abakaliki. These organisms were characterized and identified to species level using standard identification technique. Sensitivity studies were carried out on the test organisms using disc diffusion method and later the organisms were characterized phenotypically for ESBL production using the Double Disc Synergy Test (DDST). A preliminary molecular characterization of the ESBL producing isolates were further carried out based on the evaluation of their plasmid profile via agarose gel electrophoresis. The over-all result of the study revealed that the prevalence of ESBL producing organisms was high 18 (16.5%) in our environment. The rate of occurrence varied within the two hospitals with 11 (23.9%) from FMC (urine 2 (18.2%), blood 5 (35.7%), wound 3 (30%) semen 1 (33.3%) and non was isolated from sputum while 7(11.1%) were from EBSUTH (urine 2(9.5%), blood 3 (21.4%), wound 2 (18.2%) respectively and non was isolated from sputum and semen. The plasmid profile studies revealed the presence of low molecular weight plasmid DNA within the ranges of 21.3-29.4 kb.

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