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Research Journal of Microbiology
Year: 2008  |  Volume: 3  |  Issue: 6  |  Page No.: 457 - 465

Inhibitory Effect of Caffeine on Growth of Various Bacterial Strains

Swati Sucharita Dash and Sathyanarayana N. Gummadi    

Abstract: The study was undertaken to investigate the effect of caffeine (1, 3, 7- trimethylxanthine) on growth, morphology and viability of caffeine degrading Pseudomonas sp. and other non caffeine degrading bacterial strains. Growth, morphology and cell viability of the bacterial strains were studied in caffeine medium, minimal medium without caffeine and in minimal medium with caffeine added at log phase of growth. The caffeine degrading Pseudomonas sp. achieved a maximum cell dry weight of 1.1 g L-1 after caffeine addition at log phase of growth without any change in morphology. The growth and viability of E. coli DH5α and other bacterial strains was greatly reduced upon addition of caffeine at log phase of growth. E. coli DH5α strain formed long filamentous cells on caffeine exposure and lysis occurred for other Gram negative bacterial species and Bacillus subtilis. E. coli DH5α transformed with plasmid from caffeine degrading Pseudomonas sp. was found to be tolerant to caffeine. This study shows the susceptibility of non caffeine degrading bacterial strains to caffeine and will be helpful in understanding the basics of the evolution and survival of xenobiotic degrading strains in nature.

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