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Research Journal of Microbiology
Year: 2008  |  Volume: 3  |  Issue: 5  |  Page No.: 319 - 325

Microbial Growth and Chemical Analysis of Mineral Contents in Bottled Fruit Juices and Drinks in Riyadh, Saudi Arabia

Suaad S. Alwakeel and Eman Abdullah Hamad Al-Humaidi    

Abstract: This study aimed to determine the clinically important levels of minerals in bottled fruit juices and drinks and to determine the microbial contamination of commercially available bottled fruit juices and drinks from different supermarkets in Riyadh, Saudi Arabia. Commercially available bottled fruit juices and drinks were brought from different supermarkets in Riyadh, were examined microbiologically and mineral contents were determined by atomic absorption spectrophotometry. A total of 150 specimens (3 replicates of a total 50 samples) were examined for microbial growth on six different culture media (BAP, NA, MacConkey, CAP, Salmonella Agar and PDA). A total of 43 (28.7%) different colonies were seen on different fruit juices. Bacillus cereus was the most common isolate in all types of fruit juices. Other isolates included Bacillus subtilis, Bacillus polymyxa, Chryseomonas luteola, Tatumella ptyseos, Streptococcus lactis and Candida sp. None of the specimens taken from softdrinks and power drinks showed any microbial growth after incubation for 48 h in all six environmental plates used. Specimens from mixed juice with milk showed microbial colonies in 3 out of 10 specimens with Lactobacillus sp., Streptococcus lactis and Lactobacillus casei. The mineral contents of 8 specimens of fruit juices had iron content within the maximum allowed concentration. As to potassium content, 7 of 8 (87.5%) of the samples had potassium content >10 ppm. Five of 8 (62.5%) samples had sodium content >20 ppm, 7 of 8 (87.5%) had aluminum content >0.2 ppm, 4 of 8 (50%) had lithium content >0.2 ppm, 7 of 8 (87.5%) had magnesium content >30 ppm, 4 of 8 (50%) had manganese content >20 ppm, all 8 contained lead >0.2 ppm and 7 of 8 (87.5%) have zinc content >5 ppm. Commercially sold fruit juices in Riyadh, Saudi Arabia should be further investigated and regulated since they contain dangerous organisms and minerals which are toxic to the body.

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