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Research Journal of Microbiology
Year: 2008  |  Volume: 3  |  Issue: 4  |  Page No.: 282 - 287

Production of Catechol-type of Siderophores by Rhizobium sp. Isolated from Stem Nodules of Sesbania procumbens (Roxb.) W and A

M. Sridevi, K.G. Kumar and K.V. Mallaiah    

Abstract: The Rhizobium sp. isolated from stem nodules of Sesbania procumbens (Roxb.) W and A was studied for its ability to produce siderophores on Chrome-Azurol S agar medium. The symbiont was able to produce catechol-type of siderophores in culture after 4 h of incubation. Maximum siderophore production was observed after 24 h. Carbon and nitrogen sources greatly influence the siderophore production. Among the carbon and nitrogen sources, mannitol (2%) and glutamine (0.1%) were found to increase the siderophore production. Thin Layer Chromatography (TLC) of the siderophore extract showed the presence of 2, 3-Dihydroxy Benzoic Acid (DHBA) and 3, 5-DHBA. Arginine, glutamine and proline were identified as conjugated amino acids of siderophore extract.

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