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Research Journal of Microbiology
Year: 2008  |  Volume: 3  |  Issue: 3  |  Page No.: 163 - 168

Isolation and Identification of Lactic Acid Bacteria and Yeast from Raw Milk in Khartoum State (Sudan)

Zeinab A.M. Elgadi, Warda S. Abdel Gadir and Hamid A. Dirar    

Abstract: Fifty four raw milk samples were collected from cows (farms and venders), goats, ewes and camels of different areas of Khartoum state and microbiologically analyzed. Enumeration and isolation were carried out anaerobically at 37°C on MRS and M17 for lactococci and lactobacilli, respectively, aerobically on PDA at 25°C for yeasts and on nutrient agar for total viable count at 37°C. Sixty three MRS and M17 isolates and six PDA Isolates were purified and kept at 4°C for further identification. The presence of Lactic Acid Bacteria (LAB) and yeast was confirmed by colonial morphology, microscopy in addition to other biochemical tests. The ability of streptococci to ferment and assimilate sugars was carried out using the API kits. The results obtained showed that the milk samples contained lactobacilli and lactococci in the range of 3.50-6.30 and 3.48-6.21 log mL-1, respectively, yeast in the range of 2.00-3.95 log mL-1 and the total viable count in the range of 3.48-7.98 log mL-1. Lactic acid bacteria isolated from milk samples belonged to lactobacillus and streptococcus genera. The homofermentative lactobacilli from cows and camel milk were tentatively identified as Lactobacillus plantarum and Lb. acidophilus, whereas the hetero fermentative ones from cows, goats and ewes milk were found to be Lb. fermentum. The homofermentative streptococci isolated from all milk samples were tentatively identified as Streptococcus cremoris and Streptococcus lactis, whereas the only heterofermentative strain from camel milk was found to be Leuconostoc lactis. Yeasts which were only isolated from cow`s milk, were identified as Debaryomyces hansenii (4 strains), Kluveromyces lactis (one strain) and Saccharomyces rouxii (one strain).

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