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Research Journal of Microbiology
Year: 2008  |  Volume: 3  |  Issue: 3  |  Page No.: 122 - 128

Phenazine Pigments from Pseudomonas aeruginosa and Their Application as Antibacterial Agent and Food Colourants

Samanta Saha, R. Thavasi and S. Jayalakshmi    

Abstract: This study deals with isolation and identification of Pseudomonas aeruginosa from marine environment for phenazine pigment production. Pigment production results revealed that, the strain could able to produce two different pigments namely, pyocyanin and pyorubrin. Maximum biomass was observed at 66 h of incubation, but the pigment production seems to be continuous throughout the culture period (72 h). Antibacterial activity of the pigments was evaluated against pathogenic bacteria, maximum growth inhibitory activity was observed with pyocyanin and pyorubrin at 20 μL concentration (1.7 and 1.3 cm, respectively) against Citrobacter sp. Hemolytic activity of the pigments inferred that, hemolysis was observed with both pigments at 15, 20 and 25 μL concentration and no hemolysis was found at 5, 10 and 15 μL. The pigments were evaluated for their potential as food colourants with agar. Pleasant colouration was observed with pyocyanin and pyorubrin at 25 mg mLG-1 concentration.

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