Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Research Journal of Microbiology
Year: 2007  |  Volume: 2  |  Issue: 10  |  Page No.: 727 - 734

Effects of Extraction Methods and Age of Cells on the Whole-cell Protein Patterns of Lactobacillus

C.C. Sieo, B.T. Wong, N. Abdullah and Y.W. Ho    

Abstract: Whole-cell protein fingerprinting has been used in various studies for identification, characterization and differentiation of bacteria. It has been observed that the methods of extraction of the whole-cell protein and age of the cells used were varied in several studies. Thus, in this study, the effects of different extraction methods and age of cells on the whole-cell protein patterns were evaluated. The whole-cell proteins from Lactobacillus reuteri C1, C10 and C16, Lactobacillus salivarius I24 and Lactobacillus gallinarum I16 and I26 were extracted from 6, 12, 18 and 24-h-old cells by using lysozyme treatment, glass beads and sonication. A comparison of the whole-cell protein profiles generated showed that extraction by lysozyme treatment produced the most complete profile, with protein bands at high intensities. Glass beads and sonication could only extract major proteins. The age of cells also affected the whole-cell protein profile. Different strains were found to produce optimum results at different cell ages when extracted with the three methods.

Cited References   |    Fulltext    |   Related Articles   |   Back
 
 
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility