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Research Journal of Microbiology
Year: 2007  |  Volume: 2  |  Issue: 9  |  Page No.: 690 - 695

Mass Production of Endomopathogenic Fungus Metarhizium anisopliae (Deuteromycota; Hyphomycetes) in the Laboratory

P. Soundarapandian and R. Chandra    

Abstract: In the present study, mass production of Metarhizium anisopliae was carried out in the laboratory by using liquid and solid media. The spore count, radial growth, sporulation and biomass were maximum when M. anisopliae were cultured both czapeck dox agar and potato dextrose agar media. Hence, these two media can be used for successful mass production of fungus, M. anisopliae. Four types of solid media such as rice, maize, wheat and kodomillet were used for the mass production of M. anisopliae and highest sporulation was observed in M. anisopliae cultured in wheat (8,300x104). Hence, wheat is recommended as best solid media for the mass production of M. anisopliae. The optimum temperature and ideal pH for the mass production of M. anisopliae was found to be 25-30°C and 7.0, respectively. Various culture containers such as Erlinmayer’s flasks, petriplates and plastic bags were also used for the mass production of M. anisopliae. But very good result was obtained in High-Density Polyethylene (HDPE) bags. So this high-density polyethylene (HDPE) bags are ideal and also cost effective for the mass production of M. anisopliae.

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