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Research Journal of Microbiology
Year: 2007  |  Volume: 2  |  Issue: 9  |  Page No.: 664 - 670

Application of Box-Behnken Design for Optimization of Poly-γ-Glutamic Acid Production by Bacillus licheniformis SAB-26

Yasser R. Abdel-Fattah, Nadia A. Soliman and Mahmoud M. Berekaa    

Abstract: Optimization of poly-γ-glutamic acid (PGA) produced by the glutamate-independent Bacillus licheniformis SAB-26 has been carried out using response surface methodology. Predicted maximum PGA yield (57.9 g L-1) was calculated using non-linear optimization algorithm powered by Microsoft Excel, where the optimized medium composition was (g L-1): casein hydrolysate, 12.2; ammonium sulfate, 20; K2HPO4, 27 and KH2PO4, 27 after 72 h. A verification experiment was carried out to compare predicted and experimental results, where experimental yield reached (59.9 g L-1) in a good sign of model validity. Pulsed feeding experiment of citrate at different time intervals revealed increasing the polymer production to 88 g L-1.

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