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Research Journal of Microbiology
Year: 2007  |  Volume: 2  |  Issue: 1  |  Page No.: 66 - 73

Study of Varying Temperature and pH on Bacterial Cell Morphology of Wild Type (PY79) and Sporulation Defective Strains of Bacillus

Saira Anwar, Anjum Nasim Sabri and Shahida Hasnain    

Abstract: The present study deals with the effect of temperature (37 and 45°C) in combination with varying pH (6, 7, 8, 9) after 24 and 96 h of incubation on cell morphology, staining behavior and growth rate of PY79 (wild type) and sporulation defective mutant strains (5, 19, 96, 99) of bacillus. Strains were isolated from local polluted area around Lahore. All the strains were gram positive spore former aerobic rods. In majority of strains the optimum temperature was 37°C and pH 7. Frequencies of different cell types differ at different temperatures and at varying pH. Cell degeneration and lysis was more in sporulation defective strains (5, 19, 96, 99). Spores which were formed by the bacterial isolates 5, 19, 96 and 99 were different from wild type and were unable to germinate.

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