Abstract: Attempts have been made to quantify the heavy metal and heavy metal resistant bacteria in the two stations in Uppanar estuary (Lat 11°43’ Long 79° 49’). The distribution of selected heavy metals (Hg, Cd, Cu and Zn) and the level of metals in the sediment was in the order of Zn>Cu>Cd>Hg. The existence of profound seasonal variation in the distribution of metals and heavy metal resistant bacteria in the sediments was observed. The metals and their resistant bacteria were maximum during monsoon and minimum during summer. The higher level of metal concentration and bacterial population were recorded near common effluent discharge from SIPCOT industrial complex, as it receives more quantities of industrial waste.