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Research Journal of Microbiology
Year: 2007  |  Volume: 2  |  Issue: 3  |  Page No.: 272 - 277

Isolation and in vitro Antimicrobial Activities of Ethyl acetate Extract from Streptomyces bangladeshiensis

M. Abdul Alim Al-Bari, Alam Khan, M. Robiul Islam, M. Kudrat-E-Zahan, M.M. Sazedur Rahman, M. Anwar Ul Islam and M. Ashik Mosaddik    

Abstract: The aim of the present study was to investigate the antimicrobial activities of ethyl acetate extracts against Gram-positive and Gram-negative bacteria and fungi. An attempt has been made to investigate the indigenous drugs of choice in infectious diseases for mitigation of suffering of the vast masses of humanity. The organism, Streptomyces bangladeshiensis was isolated from a soil sample collected from Natore, Bangladesh at the depth of 0.75 m using crowded plate technique. The maximum secretion of metabolites from the strain was found at the 7th day of incubation in Czapek Dox broth (alkaline pH 8.5) medium at 32.5°C by maintaining the physicochemical factors in optimum level for the culture. In vitro antimicrobial susceptibility was determined as per National Committee for Clinical Laboratory Standards guidelines and serial dilution technique for the determination of Minimum Inhibitory Concentration (MIC) of extracts. Ethyl acetate extract from a new actinomycetes, Streptomyces bangladeshiensis, showed good antibacterial and antifungal activities against a total of 14 bacteria (5 gram positive plus 9 gram negative) and 8 fungi. The Minimum Inhibitory Concentrations (MIC) were determined and found to be 16 μg mL-1 against Bacillus subtilis, Staphylococcus aureus, Shigella dysenteriae and Aspergillus flavus while 32 μg mL-1 against Salmonella typhi, Candida albicans and Aspergillus niger. Present data shows that all the pathogenic microorganisms (Gram positive and negative bacteria and fungi) showed a substantial sensitivity towards the crude extract. But further work is necessary in order to establish the individual antibiotics present of this isolated compound.

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