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Research Journal of Microbiology
Year: 2006  |  Volume: 1  |  Issue: 5  |  Page No.: 409 - 415

Gelatin Net Hydrolyzate: A Novel Enhancer for Citric Acid Production by Aspergillus niger

Walid A. Lotfy    

Abstract: This study aimed at enhancing citric acid production on pilot scale fermentors of 3600 l capacity with 2500 l working volume by Aspergillus niger UMIP 2563.04 using Gelatin Net Hydrolyzate (GNH), a by-product of soft gelatin capsules manufacturing. The results show that the addition of GNH to the fermentation medium has shortened the fermentation period from 212 to 152 h. The A. niger strain produced 175±1 g L-1 citric acid when grown on a medium containing beet molasses (200 g L-1 total sugar) and GNH while it produced 70.65±1 g L-1 citric acid when grown on the same medium deprived from gelatin net hydrolyzate. On the basis of kinetic parameters, the efficiency of substrate uptake rate with respect to Qs and qs revealed that the strain grew significantly (p≤0.05) faster in the presence of GNH in the fermentation medium. Subsequent to the addition of GNH, the strain was significantly (p≤0.05) improved for the values of product yield coefficients (Yp/s), (Yp/x), citric acid productivity (Qp) and citric acid specific productivity (qp).

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