Search. Read. Cite.

Easy to search. Easy to read. Easy to cite with credible sources.

The Journal of General Physiology

Year: 2010  |  Volume: 135  |  Issue: 1  |  Page No.: 15 - 27

Flux regulation of cardiac ryanodine receptor channels

Y Liu, M Porta, J Qin, J Ramos, A Nani, T. R Shannon and M. Fill

Abstract

The cardiac type 2 ryanodine receptor (RYR2) is activated by Ca2+-induced Ca2+ release (CICR). The inherent positive feedback of CICR is well controlled in cells, but the nature of this control is debated. Here, we explore how the Ca2+ flux (lumen-to-cytosol) carried by an open RYR2 channel influences its own cytosolic Ca2+ regulatory sites as well as those on a neighboring channel. Both flux-dependent activation and inhibition of single channels were detected when there were super-physiological Ca2+ fluxes (>3 pA). Single-channel results indicate a pore inhibition site distance of 1.2 ± 0.16 nm and that the activation site on an open channel is shielded/protected from its own flux. Our results indicate that the Ca2+ flux mediated by an open RYR2 channel in cells (~0.5 pA) is too small to substantially regulate (activate or inhibit) the channel carrying it, even though it is sufficient to activate a neighboring RYR2 channel.

View Fulltext