Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Journal of Food Technology
Year: 2006  |  Volume: 4  |  Issue: 2  |  Page No.: 111 - 115

PCR-Based Detection of Aeromonas from Milk Samples

Porteen, K., R.K. Agarwal and K.N. Bhilegaonkar    

Abstract: A set of primers targeting 16S rRNA gene and aerolysin gene was employed to standardize PCR assay for detection of Aeromonas from milk samples. The primers used were found to be highly specific for Aeromonas spp and did not give positive result with other Gram positive and Gram-negative bacteria. The minimum detection level of PCR was found to be 102 cells -1 and 104cells -1 in case of 16S rRNA and aerolysin gene targeted assay, respectively. Suitability of the enrichment broth (Alkaline peptone water-cephalothin, APW-C) when tested to detect Aeromonas from the spiked samples gave good results on direct usage of the broth for template preparation without any subsequent treatment. The kinetics of the spiking study indicated that a minimum of 24 hrs enrichment was required for the detection of Aeromonas by cultural and PCR method. Among two PCR assays detection limits achieved by PCR targeting16S rRNA gene were better than aerolysin gene PCR assay. The results were comparable to cultural method. Examination of 50 milk samples(Pooled samples) revealed two samples to be positive by cultural method and PCR targeting 16S r RNA.Thus the standardized single –step enrichment PCR protocol holds promise as a reliable and rapid method for the detection of Aeromonas from milk samples.

Fulltext    |   Related Articles   |   Back
   
 
 
 
  Related Articles

 
 
 
 
 
 
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility