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Journal of Food Technology
Year: 2005  |  Volume: 3  |  Issue: 1  |  Page No.: 64 - 67

Production of Alpha Amylase by a Thermophilic Strain of Bacillus Licheniformis

Hamad Ashraf, Kokub Rana, Hifzah Zainab and Ikram-ul-Haq    

Abstract: The production of alpha amylase was carried out by thermophilic strains of Bacillus licheniformis. The isolated cultures were screened by solid-state fermentation using wheat bran as substrate, moistened with phosphate buffer (pH 7.5). The culture GHB8 that gave the maximum production of alpha amylase (1277 U/g/min) was used for further studies. The maximum production of alpha amylase (1630 U/g/min) was reached when wheat bran was partially replaced with cottonseed meal in the ratio of 3:1 along with 1 per cent starch. The incubation temperature 40oC was found to be optimum for the production of alpha amylase. The enzyme was most active between 60-70?C.

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