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Journal of Fisheries and Aquatic Science
Year: 2013  |  Volume: 8  |  Issue: 4  |  Page No.: 494 - 505

Optimizing the Enzymatic Hydrolysis of Skipjack Tuna (Katsuwonus pelamis) Dark Flesh Using Alcalase® Enzyme: A Response Surface Approach

Herpandi, N. Huda, A. Rosma and W.A. Wan Nadiah    

Abstract: The goal of this study was to optimize enzymatic hydrolysis of the dark flesh of skipjack tuna (Katsuwonus pelamis). Alcalase 2.4 L® was used as the hydrolytic enzyme at various concentrations (1, 1.5, 2, 2.5 and 3% w/w) at pHs from 6 to 10, temperatures from 35 to 75°C and times of 2 to 6 h. The experiment was performed following a five-level second-order central composite design with six replications at the center points. The high coefficients of determination for degree of hydrolysis (R2 = 0.8909) and free tryptophan content (R2 = 0.9732) indicate the suitability of the design for predicting the responses. The optimum hydrolytic conditions predicted by the response surface methodology were 2% Alcalase® at pH 8.86 and 65.4°C for 5.74 h. These conditions resulted in an experimental degree of hydrolysis of 20.74% and free tryptophan content of 102.23 mg kg-1.

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